THESIS
2017
x, 58 pages : illustrations (some color) ; 30 cm
Abstract
Membrane trafficking is an essential process for transporting materials in cells. It consists
of a number of trafficking pathways interacting and regulating each other forming a
sophisticated network. Despite it has been intensively studied for decades, there are still a lot
of puzzles remained unsolved. In this study, we characterize the role of MTC1, which is a gene
with unknown function and has been speculated being related to maintenance of telomere
capping, for its involvement in the membrane trafficking processes, and provide evidence to
show that it is involved in cis-Golgi associated trafficking
In a previous suppressor screening study of a trafficking mutant, vps74 arginine mutant,
our former lab member, miss Ping Yu, discovered that MTC1 is a mild temperature sensitivi...[
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Membrane trafficking is an essential process for transporting materials in cells. It consists
of a number of trafficking pathways interacting and regulating each other forming a
sophisticated network. Despite it has been intensively studied for decades, there are still a lot
of puzzles remained unsolved. In this study, we characterize the role of MTC1, which is a gene
with unknown function and has been speculated being related to maintenance of telomere
capping, for its involvement in the membrane trafficking processes, and provide evidence to
show that it is involved in cis-Golgi associated trafficking
In a previous suppressor screening study of a trafficking mutant, vps74 arginine mutant,
our former lab member, miss Ping Yu, discovered that MTC1 is a mild temperature sensitivity
(TS) suppressor of the mutant. From there, we hypothesized MTC1 may have a role in the
membrane trafficking. To investigate its role, genetic interaction and cellular physiology
experiments were conducted. The genetics study showed MTC1 is synthetic TS with four non-essential
subunits of COG complex, specific subunit of TRAPPIII, SNARE SEC22, GET
complex subunit GET3 and Golgi small GTPase YPT6, strongly indicating the involvement of
Mtc1p in traffic processes. Cellular physiology studies showed that Gas1 and CPY maturations
are defective in those TS double mutants, suggesting the role of Mtc1p is associated with Golgi
apparatus. Finally, localization studies show that Mtc1p localizes to the early Golgi
compartment, but the late Golgi, further narrowing down the role of Mtc1p to cis-Golgi
associated trafficking.
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