THESIS
2018
8 unnumbered pages, xiv, 161 pages : illustrations ; 30 cm
Abstract
Nitrofuran (NF) is a class of broad-spectrum veterinary drugs for the treatment of
microbial infections. They are highly effective in suppressing and killing most kinds of
bacteria, fungi, and protozoa, which let them to be used extensively in aquaculture and
livestock farming as growth promoters until they are observed to be carcinogenic and
mutagenic to humans. Currently, the use of NF in food-producing animals has been
banned in many countries, including China, U.S.A., and many European countries.
However, illegal use of NF exists and the toxicological mechanism of NF is poorly
understood. I investigated in this thesis for the first time the genotoxic effect of NF by
rigorously quantifying the DNA adducts formed by reacting the hydrazine-containing
metabolites of NF with aba...[
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Nitrofuran (NF) is a class of broad-spectrum veterinary drugs for the treatment of
microbial infections. They are highly effective in suppressing and killing most kinds of
bacteria, fungi, and protozoa, which let them to be used extensively in aquaculture and
livestock farming as growth promoters until they are observed to be carcinogenic and
mutagenic to humans. Currently, the use of NF in food-producing animals has been
banned in many countries, including China, U.S.A., and many European countries.
However, illegal use of NF exists and the toxicological mechanism of NF is poorly
understood. I investigated in this thesis for the first time the genotoxic effect of NF by
rigorously quantifying the DNA adducts formed by reacting the hydrazine-containing
metabolites of NF with abasic sites in DNA of bacteria and in internal organs of NF-treated
rats. Furthermore, I developed a simple and sensitive high-performance liquid
chromatography−fluorescence detection (HPLC−FLD) with automated in-injector
derivatization method for the determination of NF in food samples. Using my
developed HPLC−FLD and high-performance liquid chromatography−tandem mass
spectrometry detection method, I further investigated the uptake and metabolism of NF
in crops, which is one of the pathways that the genotoxic NFs enter human body after
they are discharged into environment. It is my belief that the results in my thesis work
have allow better understanding of the genotoxicity of NF and that my developed
HPLC−FLD should facilitate regulation and surveillance on illegal use of NF in the
food industry.
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