THESIS
2018
xv, 97 pages : illustrations (some color) ; 30 cm
Abstract
The emerging realms of bioelectronics have brought the advent of numerous therapeutic and
diagnostic biomedical devices. One of the promising example of organic bioelectronics is the
organic electrochemical transistors (OECTs). Although most of the attentions have been
dedicated to the applications of microelectrode arrays and field-effect transistors during the past
decades, OECT nevertheless offers an attractive high signal transducing capability and intrinsic
flexible mechanical property, endowing it with great benefits in interfacing with biological
milieu. Most of the biological sensing on OECT platforms, however, stayed at single-channel
recording stage. Therefore the spatial dependence electrophysiological activities were rarely
studied on OECT.
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The emerging realms of bioelectronics have brought the advent of numerous therapeutic and
diagnostic biomedical devices. One of the promising example of organic bioelectronics is the
organic electrochemical transistors (OECTs). Although most of the attentions have been
dedicated to the applications of microelectrode arrays and field-effect transistors during the past
decades, OECT nevertheless offers an attractive high signal transducing capability and intrinsic
flexible mechanical property, endowing it with great benefits in interfacing with biological
milieu. Most of the biological sensing on OECT platforms, however, stayed at single-channel
recording stage. Therefore the spatial dependence electrophysiological activities were rarely
studied on OECT.
This thesis shows an example on how to engineer multichannel OECT arrays into a versatile
two-dimensional extracellular electrophysiology recording platform for mapping cardiac action
potentials and screening for new drugs, as well as for monitoring the integrity of barrier tissues.
In the first part of the study, a 16-channel OECT array sensing platform was established for
measuring the cardiac action potential of varies cardiac cells including primary rat
cardiomyocytes and cardiomyocytes from human pluripotent stem cells (hPSC-CMs). The
speed and direction of the action potential propagation was calculated from a polynomial
surface fitted from space-time coordinates. The action potential patterns of 2D cell monolayer
and 3D microtissues displayed distinct features on OECT. Examples of drug testing was also
demonstrated on OECT array by treating two chronotropic agents on cardiac tissues. The
validation of a 64-channel OECT array for monitoring action potentials was demonstrated for
the first time. In the second part, the 16-channel OECT array was utilized to monitor the
invasive property of nasopharyngeal carcinoma on epithelial barriers. The harmonic
transconductance-based sensing on OECT revealed the distinguishable transepithelial
resistance of epithelial cells and cancer cells. Such a measurement on the 2D array furthermore
illustrated the spatial distribution of cancer cells and epithelial cells in their co-culture. This
thesis expanded the scopes of OECTs into a 2D biological environment, where the spatial
dependent electrophysiological information was able to be sensed by the OECTs. This
facilitates the further development of OECT array for pharmacological applications and
fundamental studies.
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