THESIS
2019
94 pages : illustrations (1 color) ; 30 cm
Abstract
Steviol glycoside (SG) is the non-caloric food additive extracted from stevia rebaudiana
leaves. It is a mixture of four major components, which are Rebaudioside A (RA),
Rebaudioside C (RC), Rebaudioside D (RD) and Stevioside (STV). The RD is capable
of providing 250-300 times sweetness than the sugar and giving the clean aftertaste.
The conventional approach for separating each pure rebaudioside from SG is
crystallization. However, such efficiency is limited when applying to different
rebaudiosides’ concentrations in SG. In this study, therefore, an approach of the resin-based
frontal chromatography has been investigated for RD separation in order to solve
the deficiency of crystallization technique - slow production rate and low product yield.
Several resins were attempted fo...[
Read more ]
Steviol glycoside (SG) is the non-caloric food additive extracted from stevia rebaudiana
leaves. It is a mixture of four major components, which are Rebaudioside A (RA),
Rebaudioside C (RC), Rebaudioside D (RD) and Stevioside (STV). The RD is capable
of providing 250-300 times sweetness than the sugar and giving the clean aftertaste.
The conventional approach for separating each pure rebaudioside from SG is
crystallization. However, such efficiency is limited when applying to different
rebaudiosides’ concentrations in SG. In this study, therefore, an approach of the resin-based
frontal chromatography has been investigated for RD separation in order to solve
the deficiency of crystallization technique - slow production rate and low product yield.
Several resins were attempted for the study. In which, CAD40 resin shows distinctive
capability for separating the RD from bulk SG. Samples with a high concentration of
RD were taken in the early part of breakthrough curve experiment. With this selectivity,
the pure RD is able to be separated from the mainstream under the optimized condition.
This study shows the feasibility that the rebaudioside can be extracted by using suitable
resin, which is not only achieved by the biocatalytic method – selective digestion by
the enzymes.
Post a Comment