THESIS
2020
xxi, 134 pages : illustrations (some color) ; 30 cm
Abstract
The rising demand in therapeutic biomaterial such as bioactive wound dressing material
required the research in advanced biomaterials to relief government’s financial burden and
improve human health quality. In this project, to combine antibacterial and wound healing
property from silver (Ag) and poly(lactic-co-glycolic) acid (PLGA) respectively, the silver
nanoparticle-attached PLGA nanofibers were synthesized with novel environmental-friendly
post-electrospinning treatment. The smallest uniform PLGA nanofibers were confirmed with
average diameter of 149nm from dichloromethane and ethanol mixture as least-toxic solvent.
Positive changes in diameter were shown by adjusting the applied voltage and the parameters
to produce PLGA nanofiber at 150nm, 350nm, 450nm and 550nm were stan...[
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The rising demand in therapeutic biomaterial such as bioactive wound dressing material
required the research in advanced biomaterials to relief government’s financial burden and
improve human health quality. In this project, to combine antibacterial and wound healing
property from silver (Ag) and poly(lactic-co-glycolic) acid (PLGA) respectively, the silver
nanoparticle-attached PLGA nanofibers were synthesized with novel environmental-friendly
post-electrospinning treatment. The smallest uniform PLGA nanofibers were confirmed with
average diameter of 149nm from dichloromethane and ethanol mixture as least-toxic solvent.
Positive changes in diameter were shown by adjusting the applied voltage and the parameters
to produce PLGA nanofiber at 150nm, 350nm, 450nm and 550nm were standardized. This
simple adjustment to control fiber diameter was not shown if replacing the solvent by acetone
or tetrahydrofuran. Spherical BIORIMA silver and gold nanoparticles were confirmed at the
size of 16nm and 14nm respectively. UV-Vis spectra, crystallite structure, valence band
information and molecular confirmation of silver and gold nanoparticles were also done with
different material characterization tools. To overcome the super-hydrophobicity of neat PLGA
nanofiber, the silver and gold nanoparticles attachment were conducted after calcium chloride
salt treatment to allow better physical contact. Weight percentage of silver at 7.6% was
measured in treated PLGA nanofiber for 90 minutes, meanwhile, the nanoparticle structure and
nanofiber network retained in this treatment. The 7.6% Ag-PLGA nanofibers were confirmed
with antibacterial activities on E. Coli and S. Aureus comparable to 0.254% sodium
hypochlorite. The outstanding performance suggested this novel environmental-friendly post-electrospinning
treatment allowed better control of the composite structures, enhancement in
material efficiency and least pollution to environment while preserving antibacterial activities
for biological applications. With the adoption of Au-PLGA nanofiber for the comprehensive
study of nanoparticles movement in-vivo in future, it is foreseeable the Ag-PLGA nanofibers
can benefit the public health system economically and improve human health quality.
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