THESIS
2020
ix leaves, 39 pages : illustrations (some color) ; 30 cm
Abstract
The inability of axon regeneration in adult mammalian central nervous system (CNS) has
been a major question in both fundamental and clinical neuroscience. Some regulatory
mechanisms on axon regeneration have been identified but our understanding is still limited.
To systematically explore novel targets, we tried to set up a genetic screening for axon
elongation in vitro. By using short hairpin RNA targeting Gsk3b, we successfully knocked
down the gene in cells and observed more and longer neurite outgrowth. Gsk3b knockout has
previously been shown to promote axon regeneration in vivo. By using shGsk3b as a positive
control and a software for automatic neurite quantification, we started to test a small
proportion of shRNAs from a library. Some showed potential for increasing neu...[
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The inability of axon regeneration in adult mammalian central nervous system (CNS) has
been a major question in both fundamental and clinical neuroscience. Some regulatory
mechanisms on axon regeneration have been identified but our understanding is still limited.
To systematically explore novel targets, we tried to set up a genetic screening for axon
elongation in vitro. By using short hairpin RNA targeting Gsk3b, we successfully knocked
down the gene in cells and observed more and longer neurite outgrowth. Gsk3b knockout has
previously been shown to promote axon regeneration in vivo. By using shGsk3b as a positive
control and a software for automatic neurite quantification, we started to test a small
proportion of shRNAs from a library. Some showed potential for increasing neurite length
and future experiments are needed to verify this. Together, these data indicate that we have
introduced an in vitro model for genetic screening of axon elongation.
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