THESIS
2021
1 online resource (xxxiii, 230 pages) : illustrations (some color)
Abstract
The world is facing multiple health challenges, including threats of pathogenic bacteria. Although various methods have been applied for bacterial analysis, the complicated protocols and high false positive chances hamper their efficiency. Moreover, over reliance on antibiotics to treat infectious diseases leads to irreversible antibiotics resistances. These signs direct to the PhD Thesis -- LEE Mei Suet importance and urgency for developing alternative and effective methods for bacterial discrimination and bacterial elimination.
Fluorescence shows its uniqueness of high sensitivity, selectivity and non-invasiveness among various analytical techniques. Although traditional fluorophores are widely employed, they display aggregation-caused quenching (ACQ) effect, which greatly hamper the...[
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The world is facing multiple health challenges, including threats of pathogenic bacteria. Although various methods have been applied for bacterial analysis, the complicated protocols and high false positive chances hamper their efficiency. Moreover, over reliance on antibiotics to treat infectious diseases leads to irreversible antibiotics resistances. These signs direct to the PhD Thesis -- LEE Mei Suet importance and urgency for developing alternative and effective methods for bacterial discrimination and bacterial elimination.
Fluorescence shows its uniqueness of high sensitivity, selectivity and non-invasiveness among various analytical techniques. Although traditional fluorophores are widely employed, they display aggregation-caused quenching (ACQ) effect, which greatly hamper their bioimaging applications. In contrast, aggregation-induced emission luminogens (AIEgens) are non-emissive in dilute solution but emit strongly in aggregates. The opposite performance of AlEgens show their great potentials in realizing the processes that cannot be accessed by ACQ dyes, thereby opening new avenues for fluorescent bio-innovations. In particular, AIEgens are potential candidates in alternative bacterial detection and antibacterial therapy development.
In this thesis, I have explored different AIlEgens as excellent bacterial targeting and antibacterial agents. In Chapter II, I explored a water-soluble near-infrared emissive AIEgen for ultrafast Gram-positive bacterial detection and killing. This AIEgen was further extended to investigate specific targeting and killing to intracellular bacteria in Chapter III. Regarding to initial strengths of natural fluorophores in bio-applications, I further explored two AIEgens from herbs for bacterial discrimination and elimination, including biofilm bacteria and bacterial infection treatment (Chapter IV and V). In Chapter VI, two AlEgens were designed for bacterial aggregation, as well as excellent reactive oxygen species generation under sunlight for effective bacterial killing.
The outcomes of this thesis work are anticipated to benefits both the fundamental studies and real practical utilization of AIEgens with various properties for excellent pathogenic bacterial detection and antibacterial therapy.
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