THESIS
2021
1 online resource (xiii, 127 pages) : illustrations (some color)
Abstract
Over the past a few years, biosensors have been widely used in biomedical detection and
environmental monitoring due to their advantages of high sensitivity and good selectivity.
However, most biosensor works are still in the “proof-of-concept” phase. There are still great
challenges moving from the lab to the field. This thesis work explores the feasibility of transferring
the benchtop methods to the practical integrated biosensors for portable on-site detection.
In the first work, a new integrated electrochemical magnetoimmunosensor (EC-MIS) based
on graphene and gold nanoparticles for interleukin-6 (IL-6) detection is described. An gold nanoparticles/graphene-modified electrode is fabricated to increase the sensitivity. Second, we employ
magnetic beads in immunoassay. Combining these...[
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Over the past a few years, biosensors have been widely used in biomedical detection and
environmental monitoring due to their advantages of high sensitivity and good selectivity.
However, most biosensor works are still in the “proof-of-concept” phase. There are still great
challenges moving from the lab to the field. This thesis work explores the feasibility of transferring
the benchtop methods to the practical integrated biosensors for portable on-site detection.
In the first work, a new integrated electrochemical magnetoimmunosensor (EC-MIS) based
on graphene and gold nanoparticles for interleukin-6 (IL-6) detection is described. An gold nanoparticles/graphene-modified electrode is fabricated to increase the sensitivity. Second, we employ
magnetic beads in immunoassay. Combining these two features on a microfluidic device enables
a potential portable detection on IL-6. A linear range from 0.97 to 250 pg/mL and a limit of
detection (LoD) of 0.412 pg/mL are obtained.
The second work is the first integrated and fully portable microbial whole-cell biosensing
platform for cadmium and lead detection. Escherichia coli (E. coli) cells are transformed. The
optics of the platform are designed to minimize the aberration with good spectral resolution, while
the electronics are designed to limit the noise. Experiments show the platform has limit of detection
of 518 ppb for Pb
2+ and 44.8 ppb for Cd
2+.
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