THESIS
2021
1 online resource (viii, 35 pages) : illustrations (chiefly color)
Abstract
Hemogenic endothelial cells (HECs) refer to the endothelial cells that have the potential to become hematopoietic stem cells/ progenitor cells that can undergo a cell behavior called endothelial hematopoietic transition (EHT) in zebrafish. The budding HSPCs from the aorta later will differentiate into multiple blood lineages and continuously replenish hematopoietic system. It is of great importance to understand the fate commitment and development of the HECs. However, the complex mechanism determining the HECs and conventional endothelial cells (ECs) fate has never been delineated very clearly. So far, we know that etv2-notch signaling axis is essential for the HECs fate commitment and my research aims to perfect this network.
Jam1a is a cell-cell adhesion molecule that are expressed i...[
Read more ]
Hemogenic endothelial cells (HECs) refer to the endothelial cells that have the potential to become hematopoietic stem cells/ progenitor cells that can undergo a cell behavior called endothelial hematopoietic transition (EHT) in zebrafish. The budding HSPCs from the aorta later will differentiate into multiple blood lineages and continuously replenish hematopoietic system. It is of great importance to understand the fate commitment and development of the HECs. However, the complex mechanism determining the HECs and conventional endothelial cells (ECs) fate has never been delineated very clearly. So far, we know that etv2-notch signaling axis is essential for the HECs fate commitment and my research aims to perfect this network.
Jam1a is a cell-cell adhesion molecule that are expressed in endothelial cells, epithelial cells and pronephros in zebrafish. A study indicated the role of jam1a in HECs specification. It suggested that the jam1a provide close interactions to another JAM family member on somitic cells, making the cell accessible to the notch ligand therefore cells with activated notch signaling can be committed into HECs. In my study, I further prove that jam1a is indispensable for HECs formation due to runx1 expression level decrease in both jam1a morphant and mutant. However, it remains unclear if jam1a take part in the HECs regulation in other way expect for its intrinsic feature as an adhesion molecule. Some studies indicate that jam1a may also be regulated by etv2. Therefore, more investigations should be conducted exploring the relationship between etv2 and jam1a.
Post a Comment