THESIS
2022
1 online resource (1 volume (unpaged)) : illustrations (some color)
Abstract
Eukaryotic translation is important, complex and tightly regulated process. In eukaryotes, the place for
transcription to take place is nucleus, and the place for translation to take place is cytoplasm. The
eukaryotic transcription and eukaryotic translation are not synchronized, gene is first transcribed into
mRNA in nucleus then mRNA translated into amino acid chains in cytoplasm. In the transcription
process, RNA is transcribed from DNA template and maturated by addition of 5’cap and
polyadenylation tailing at 3’ terminus. In the normal maturation step, canonical polyadenylate
polymerase (PAP) generates polyA tail at 3’terminus of mRNA. Non canonical PAP can decorate
mRNA tailing to increase the diversity. RNA tailing takes a lead in regulating translation process of
mRNA and prolong...[
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Eukaryotic translation is important, complex and tightly regulated process. In eukaryotes, the place for
transcription to take place is nucleus, and the place for translation to take place is cytoplasm. The
eukaryotic transcription and eukaryotic translation are not synchronized, gene is first transcribed into
mRNA in nucleus then mRNA translated into amino acid chains in cytoplasm. In the transcription
process, RNA is transcribed from DNA template and maturated by addition of 5’cap and
polyadenylation tailing at 3’ terminus. In the normal maturation step, canonical polyadenylate
polymerase (PAP) generates polyA tail at 3’terminus of mRNA. Non canonical PAP can decorate
mRNA tailing to increase the diversity. RNA tailing takes a lead in regulating translation process of
mRNA and prolonging mRNA half-life. Previous studies have been discovered that guanylation at
mRNA tailling can shield mRNA from rapid decay. The reason why we start this project is to discover
the mechanism of guanylation on mixed tail of mRNA. In this project, guanylation at mixed tailing
decrease the translation efficiency and half-life of mRNA. Guanylation mechanism can be hijacked
when virus infected. Therefore, we proposed that cellular self-defense might be the explanation. For the
reason of protection, RNA protein kinase (PKR) is activated for apoptosis. Before the synthetic mRNAs
enter cell to affect translation process, they are already discriminated by the cell.
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