Ethylene is a major plant hormone that plays an important role in virtually all physiological events in plant, yet the underlying molecular mechanism is very controversial. Alternative ethylene signaling pathway was proposed based on the evidence of ethylene-mediated gravitropism and ethylene-regulated blotting time results. In our quantitative proteomic studies, Nitrogen 15 metabolic peptide labeling strategies have been successfully applied to measure differentially regulated protein and peptide abundance and have been proven to be versatile and repeatable in biological discoveries. The Arabidopsis double mutant ein3-1/eil1-1 was labeled and treated with ethylene and the proteomic result reveals a number of interesting phosphopeptides that are up or down-regulated by ethylene evening...[ Read more ]

Ethylene is a major plant hormone that plays an important role in virtually all physiological events in plant, yet the underlying molecular mechanism is very controversial. Alternative ethylene signaling pathway was proposed based on the evidence of ethylene-mediated gravitropism and ethylene-regulated blotting time results. In our quantitative proteomic studies, Nitrogen 15 metabolic peptide labeling strategies have been successfully applied to measure differentially regulated protein and peptide abundance and have been proven to be versatile and repeatable in biological discoveries. The Arabidopsis double mutant ein3-1/eil1-1 was labeled and treated with ethylene and the proteomic result reveals a number of interesting phosphopeptides that are up or down-regulated by ethylene evening in the absent of the two key ethylene signaling components (EIN3 and EIL1) down-stream of EIN2. This data again, supports the high possibility of a post-translational modification network of the ethylene signaling, a more realistic picture of ethylene signaling than the simple traditional linear signaling model.