THESIS
2014
viii, 34 pages : illustrations (some color) ; 30 cm
Abstract
Myeloid cells, composed of granulocytes and monocytes, have very significant roles in the
immune response and tissue remodeling. All myeloid cells are derived from hematopoiesis.
Similar to mammals, zebrafish hematopoiesis consists of two waves, primitive hematopoiesis
and definitive hematopoiesis. These two waves take place in different locations at different time
course. Primitive hematopoiesis is a transient wave and takes place in the early stage. It mainly
gives rise to myeloid cells and erythrocytes. Definitive hematopoiesis can generate
hematopoietic stem cells, which can give rise to all kinds of cells of blood lineage including
myeloid cells, erythrocytes and lymphocytes. The macrophages generated by hematopoiesis
will migrate into different organs to become tissue resi...[
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Myeloid cells, composed of granulocytes and monocytes, have very significant roles in the
immune response and tissue remodeling. All myeloid cells are derived from hematopoiesis.
Similar to mammals, zebrafish hematopoiesis consists of two waves, primitive hematopoiesis
and definitive hematopoiesis. These two waves take place in different locations at different time
course. Primitive hematopoiesis is a transient wave and takes place in the early stage. It mainly
gives rise to myeloid cells and erythrocytes. Definitive hematopoiesis can generate
hematopoietic stem cells, which can give rise to all kinds of cells of blood lineage including
myeloid cells, erythrocytes and lymphocytes. The macrophages generated by hematopoiesis
will migrate into different organs to become tissue resident macrophages, such as microglia in
the brain.
The origin of tissue resident macrophages is an interesting research field to investigate. Myeloid
cell fate mapping in mice has been conducted by many groups to figure out which
hematopoietic wave tissue resident macrophages are derived from. However, there has not been
a consensus in this issue yet, and it still remains an open question. We tried to label different
waves of myeloid cells in zebrafish embryos and observe their distribution in the adult organs.
Two transgenic lines Tg(coro1a:CreER
T2) and Tg(coro1a:LOXP-DsRedx-LOXP-eGFP) were
generated, and this Cre-lopx system can permanently convert Dsred positive myeloid cells into
GFP positive myeloid cells. Taking the advantage of model system zebrafish, we can label one
wave of myeloid cells with different methods. Preliminary data show that controlling the time
of labelling using Tg(coro1a:CreER
T2) line or controlling the location of labelling using
(hsp70:mCherry-T2a-CreER
T2)
#12 line with a local IR laser induction may effectively label
myeloid cells at different time course or in different locations. Therefore, it is shown this system
can be applied in the myeloid cell fate mapping experiment in zebrafish.
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