THESIS
2016
xxi, 173 pages : illustrations (some color) ; 30 cm
Abstract
In this study, a dispersed mobile-phase countercurrent chromatography (DMCC)
method has been developed to facilitate scale-up of the countercurrent distribution process.
Efficient solute exchange between the two immiscible phases within a series of column is
achieved by mechanical dispersion of an influx of mobile phase into an upward stream of
small droplets travelling upward through a column of stationary phase. The diameter, length
and the number of columns can be varied to match the needs of separation.
Wogonin is one of the major flavonoids in Scutellaria baicalensis. It exhibits
anti-inflammatory, anxiolytic, neuro-protective, anti-viral and anti-cancer effects. Its multiple
pharmacological effects, methods of extraction, purification and organic synthesis have been
widel...[
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In this study, a dispersed mobile-phase countercurrent chromatography (DMCC)
method has been developed to facilitate scale-up of the countercurrent distribution process.
Efficient solute exchange between the two immiscible phases within a series of column is
achieved by mechanical dispersion of an influx of mobile phase into an upward stream of
small droplets travelling upward through a column of stationary phase. The diameter, length
and the number of columns can be varied to match the needs of separation.
Wogonin is one of the major flavonoids in Scutellaria baicalensis. It exhibits
anti-inflammatory, anxiolytic, neuro-protective, anti-viral and anti-cancer effects. Its multiple
pharmacological effects, methods of extraction, purification and organic synthesis have been
widely investigated. Two methods for organic synthesis of wogonin have been proposed, both
of which contain contaminating side products (oroxylin A or 6-chlorowogonin) that are
difficult to remove. Although wogonin can be crystallized from the crude extracts of
Scutellaria baicalensis, product yield is very low. Another proposed purification method
employs high speed countercurrent chromatography. However, in this case requirement of a
centrifugal process renders the method costly and difficult to scale up.
By DMCC, the fractionated solutes can be collected from the eluate from the
columns, or from drainage of the stationary phase in individual columns at the end of a
chromatographic run. The DMCC system developed in the present study has opened up a
useful approach to the large scale purification of wogonin from Scutellaria baicalensis for
anti-cancer and anxiolytic applications.
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