THESIS
2017
xviii, 103 pages : color illustrations ; 30 cm
Abstract
Lateral flow immunoassay is a rapid diagnostic point-of-care device prevalently used
in different areas especially in clinical diagnosis. Traditional detection methods
take few hours for the analysis that is time consuming, lab intensive and inefficient.
Lateral flow immunoassays are able to give the results within minutes and do not
require skilled technicians to perform the test because of the design of easy read out.
In view of the disadvantages of the common colorimetric signaling molecules such as
low sensitivity and only qualitative/semi-quantitative results could be achieved. A
novel signaling molecule dye-doped silica nanoparticles was synthesized by reverse
micro-emulsion. The color of the silica nanoparticles was physically entrapped and
no dye leakage was observed af...[
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Lateral flow immunoassay is a rapid diagnostic point-of-care device prevalently used
in different areas especially in clinical diagnosis. Traditional detection methods
take few hours for the analysis that is time consuming, lab intensive and inefficient.
Lateral flow immunoassays are able to give the results within minutes and do not
require skilled technicians to perform the test because of the design of easy read out.
In view of the disadvantages of the common colorimetric signaling molecules such as
low sensitivity and only qualitative/semi-quantitative results could be achieved. A
novel signaling molecule dye-doped silica nanoparticles was synthesized by reverse
micro-emulsion. The color of the silica nanoparticles was physically entrapped and
no dye leakage was observed after surface modification. The optimal size of the
dye-doped silica nanoparticles was found to be 77.25 nm for the application on the
lateral flow immunoassays. The performance of the lateral flow immunoassays
using silica nanoparticles outweighed all the existing tests with an extremely low detection limit (5 ng/mL). A multiplex detection lateral flow immunoassay was also
developed using two different colored dye-doped silica nanoparticles at the same
conjugate pad. According to the statistical pooled variance t-test, there was no
statistical difference between the intensities of the test lines from the single color
system and the dual color system.
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