THESIS
1995
x, 56 leaves : ill. (some col.) ; 30 cm
Abstract
Localized Ca
2+ signal has been suspected to play an important role in regulating cytokinesis, but so far the evidence has been indirect and controversial. In this study, we combined the usage of a sensitive fluorescence Ca
2+ indicator and laser scanning confocal microscope, and were able to demonstrate the existence of a spatially and temporally localized elevation in intracellular free calcium concentration ([Ca
2+]i) in the cleavage furrow region in zebrafish embryo. Results of this study also showed that this transient elevation in [Ca
2+]
i is necessary for the onset of cytokinesis. The source of Ca
2+ contributing to this localized elevation of [Ca
2+]
i is mainly from intracellular Ca
2+ stores released through InsP
3 receptors. Results of this study strongly support the hypothesis that...[
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Localized Ca
2+ signal has been suspected to play an important role in regulating cytokinesis, but so far the evidence has been indirect and controversial. In this study, we combined the usage of a sensitive fluorescence Ca
2+ indicator and laser scanning confocal microscope, and were able to demonstrate the existence of a spatially and temporally localized elevation in intracellular free calcium concentration ([Ca
2+]i) in the cleavage furrow region in zebrafish embryo. Results of this study also showed that this transient elevation in [Ca
2+]
i is necessary for the onset of cytokinesis. The source of Ca
2+ contributing to this localized elevation of [Ca
2+]
i is mainly from intracellular Ca
2+ stores released through InsP
3 receptors. Results of this study strongly support the hypothesis that a localized Ca
2+ signal plays an important role in regulating cytokinesis.
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