THESIS
1995
xii, 58 leaves : ill., photos. ; 30 cm
Abstract
Two multidrug resistant human acute lymphocytic leukemia cell lines (L
l00 and L
1000) were developed by exposing parental cells (L
0) to progressively increased concentrations of vincristine sulfate (VCR). These cells have a novel multilobulated nucleus and a distinct microtubule (MT) organization that may be due to the perturbation of the cytoskeleton induced by VCR. The expression of MT and microtubule-associated proteins (MAPS) of the L
100 and the L
100 cells were compared to that of the Locells. A 28 KD protein of p1 6.9 and a 31 KD protein of pI14.4 were found to be overexpressed in L
1000 and L
s1000 cells. Moreover, a 38 KD protein was found to be expressed in L
0 cells but not can be detected in L
100 and L
1000 cells. The distinct MT organization and nuclear morphology of these cells m...[
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Two multidrug resistant human acute lymphocytic leukemia cell lines (L
l00 and L
1000) were developed by exposing parental cells (L
0) to progressively increased concentrations of vincristine sulfate (VCR). These cells have a novel multilobulated nucleus and a distinct microtubule (MT) organization that may be due to the perturbation of the cytoskeleton induced by VCR. The expression of MT and microtubule-associated proteins (MAPS) of the L
100 and the L
100 cells were compared to that of the Locells. A 28 KD protein of p1 6.9 and a 31 KD protein of pI14.4 were found to be overexpressed in L
1000 and L
s1000 cells. Moreover, a 38 KD protein was found to be expressed in L
0 cells but not can be detected in L
100 and L
1000 cells. The distinct MT organization and nuclear morphology of these cells may be due to the differential expression of the MAPS. The differential expression of these MAPS is correlated to the multidrug resistance (MDR) phenotype of these cells. These data suggest that these MAPS may be associated with the mechanism of MDR.
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