THESIS
1997
xii, 90 leaves : ill., col. photos ; 30 cm
Abstract
The genus Alexandrium contains many of the most common toxic dinoflagellate species; however, many of this globally distributed genus have similar morphology. Morphological and biochemical methods such as monoclonal antibodies, isozymes as well as fluorescent characters have been employed for species detection and identification. Recently, molecular methods were applied to study the distributed populations of this toxic dinoflagellate. In the present study, PCR was performed by using established primers to isolate fragment of the large subunit ribosomal RNA from two local strains CCMP1493 of the toxic Alexandrium catenella. (isolated from Dai Ya Bay, South China) and Hong Kong sp. A. catenella (isolated from Hong Kong). The PCR products of CCMP1493 and H.K. A. catenella consisted of two...[
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The genus Alexandrium contains many of the most common toxic dinoflagellate species; however, many of this globally distributed genus have similar morphology. Morphological and biochemical methods such as monoclonal antibodies, isozymes as well as fluorescent characters have been employed for species detection and identification. Recently, molecular methods were applied to study the distributed populations of this toxic dinoflagellate. In the present study, PCR was performed by using established primers to isolate fragment of the large subunit ribosomal RNA from two local strains CCMP1493 of the toxic Alexandrium catenella. (isolated from Dai Ya Bay, South China) and Hong Kong sp. A. catenella (isolated from Hong Kong). The PCR products of CCMP1493 and H.K. A. catenella consisted of two bands, not a single band which opposed to published data on other dinoflagellates. Both PCR products of A. catenella were isolated and sequenced. The major PCR product of HK A.catenella had 6 bp different from the CCMP1493. Additionally, the sequence data of CCMP1493 and HK A catenella were compared with other published LsU RNA gene sequence data of Alexandrium species from Korea, Japan, North America. CCMP1493 and HK A. catenella had a greater similarity to the isolates of Korea and Japan than to A. catenella from North America.
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