THESIS
1998
xii, 115 leaves : ill. ; 30 cm
Abstract
Pathogenesis-related (PR) type 5 proteins are a family of pathogen-induced proteins occurring in both monocotyledous and dicotyledous plants. Two cDNAs, WRTLP1 and WRTLP2, encoding a PR-5 protein from the leaves of winter rye had been isolated. From sequence analysis, both cDNAs have the same length of open reading frame of 522 nucleotides, encoding an identical mature protein of 153 amino acids and a putative signal peptide of 20 amino acid residues. The deduced amino acid shows high sequence similarity with PR-5 proteins from leaves of other monocotyledons such as barley, wheat, oat, rice and maize so far reported. Two genomic clones WRGTLP3 and WRGTLP4 have also been isolated. The sequence alignment shows that WRTLP2 is derived from WRGTLP3 while WRGTLP4 encodes an isoform of WRTLP2....[
Read more ]
Pathogenesis-related (PR) type 5 proteins are a family of pathogen-induced proteins occurring in both monocotyledous and dicotyledous plants. Two cDNAs, WRTLP1 and WRTLP2, encoding a PR-5 protein from the leaves of winter rye had been isolated. From sequence analysis, both cDNAs have the same length of open reading frame of 522 nucleotides, encoding an identical mature protein of 153 amino acids and a putative signal peptide of 20 amino acid residues. The deduced amino acid shows high sequence similarity with PR-5 proteins from leaves of other monocotyledons such as barley, wheat, oat, rice and maize so far reported. Two genomic clones WRGTLP3 and WRGTLP4 have also been isolated. The sequence alignment shows that WRTLP2 is derived from WRGTLP3 while WRGTLP4 encodes an isoform of WRTLP2. From the comparison of ORF between WRTLP2 and WRGTLP3, it is clear that there is no intron present in the PR-5 gene. The WRTLP2 in GST fusion protein expression vector was overexpressed in E. coli strain LC137. The crude cell extract and purified protein were used to test for the antifungal activity. Preliminary result showed that the purified WRTLP2 protein was inhibitory to fungal growth under in vitro condition.
Post a Comment