THESIS
1999
xi, 102 leaves : ill. (some col.) ; 30 cm
Abstract
Retinoic acid (RA) has been suggested to play an important role in CNS development but little is known about the molecular mechanism of RA-induced neuronal differentiation. A human embryonic teratocarcinoma cell line (NT2/Dl) serves as a good model system to study neuronal differentiation in the human CNS. RAP-PCR was used in our laboratory to identify candidate genes involved in RA-induced neuronal differentiation. One of the RA-regulated gene identified, clone 37.6, encodes a partial cDNA sequence of a human nuclear DEAD box protein p72. The mRNA expression of p72 was demonstrated to be down-regulated during the RA-induced neuronal differentiation of NT2/D1 cells....[
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Retinoic acid (RA) has been suggested to play an important role in CNS development but little is known about the molecular mechanism of RA-induced neuronal differentiation. A human embryonic teratocarcinoma cell line (NT2/Dl) serves as a good model system to study neuronal differentiation in the human CNS. RAP-PCR was used in our laboratory to identify candidate genes involved in RA-induced neuronal differentiation. One of the RA-regulated gene identified, clone 37.6, encodes a partial cDNA sequence of a human nuclear DEAD box protein p72. The mRNA expression of p72 was demonstrated to be down-regulated during the RA-induced neuronal differentiation of NT2/D1 cells.
In this study, the full length cDNA sequence of p72 was obtained by screening a cDNA library prepared from undifferentiated NT2/Dl cells. p72 was found to be down-regulated during the neuronal differentiation of SH-SY5Y cells upon treatment with either RA or TPA. Similar profile of p72 regulation was observed following NGF-induced differentiation of PC12 cells. p72 mRNA was shown to be expressed in astrocytes, cortical neurons and cerebellar granule neurons. The cDNA fragment encoding rat p72 was obtained by RT-PCR and Northern blot analysis showed that p72 mRNA was widely expressed in different tissues with prominent expression in brain, eye and testis. p72 was highly expressed in brain, spinal cord, muscle and liver during embryonic stages followed by down-regulation after birth. Similar studies performed in chick demonstrated that p72 was regulated in a tissue-specific manner during development.
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