THESIS
2005
xiv, 100 leaves : ill. (some col.) ; 30 cm
Abstract
An Arabidopsis mutant, egy1-1 (e̲thylene-dependent shoot g̲ravitropism deficiency and y̲ellow-green) was identified and characterized to have a dual phenotype, reduced chlorophyll accumulation and abnormal hypocotyls gravicurvature. The mutant was caused by a 10-bp deletion in edgy1 gene, which encodes a 59-kDa metalloprotease that contains eight trans-membrane domains at its C-terminus. Egy1 gene is transcribed ubiquitously in various organs, and its protein accumulation varies from organ to organ, being most prominent in leaf and inflorescence stem. In root, the high promoter activity, low steady-state mRNA and protein level reveal that post-transcriptional regulation plays an important role. Common environmental stresses serve to down-regulate promoter activity, while affect little i...[
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An Arabidopsis mutant, egy1-1 (e̲thylene-dependent shoot g̲ravitropism deficiency and y̲ellow-green) was identified and characterized to have a dual phenotype, reduced chlorophyll accumulation and abnormal hypocotyls gravicurvature. The mutant was caused by a 10-bp deletion in edgy1 gene, which encodes a 59-kDa metalloprotease that contains eight trans-membrane domains at its C-terminus. Egy1 gene is transcribed ubiquitously in various organs, and its protein accumulation varies from organ to organ, being most prominent in leaf and inflorescence stem. In root, the high promoter activity, low steady-state mRNA and protein level reveal that post-transcriptional regulation plays an important role. Common environmental stresses serve to down-regulate promoter activity, while affect little in steady-state mRNA and protein level. However, the inconsistency between steady-state mRNA and protein level implicates post-translational regulation of egy1 gene in the process of leaf development and senescence. The absence of EGY1 protein in mutant does not result in any difference of CAB and Rubisco activase content in leaf compared with wild type plants. Besides chloroplasts, EGY1 probably also associates with amyloplasts, and immunoblot analysis revealed that EGY1 might involve in hypocotyls gravitropism in light-grown seedlings.
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