THESIS
2019
ix, 63 pages : color illustrations ; 30 cm
Abstract
Drug resistance is one of the greatest impediments in the treatment of cancer that adds a layer
of challenge in anticancer drug development. A number of chemotherapeutic drugs, such as
Cisplatin and doxorubicin, already exist and are widely used in the market, but not without the
same challenge of resistance. Here, we iterate a spectrum of intracellular mechanisms cells often
yield to gain resistance to different types of anticancer drugs, with the Primary focus on the
establishment and characterization of M2-resistance cancer cell populations and clones.
M2 is an anticancer drug candidate currently under development with a target-based mechanism.
Previous studies in our lab strongly suggest that M2 inhibits cellular proliferation by interrupting
the assembly of Minichromosome m...[
Read more ]
Drug resistance is one of the greatest impediments in the treatment of cancer that adds a layer
of challenge in anticancer drug development. A number of chemotherapeutic drugs, such as
Cisplatin and doxorubicin, already exist and are widely used in the market, but not without the
same challenge of resistance. Here, we iterate a spectrum of intracellular mechanisms cells often
yield to gain resistance to different types of anticancer drugs, with the Primary focus on the
establishment and characterization of M2-resistance cancer cell populations and clones.
M2 is an anticancer drug candidate currently under development with a target-based mechanism.
Previous studies in our lab strongly suggest that M2 inhibits cellular proliferation by interrupting
the assembly of Minichromosome maintenance protein complex (MCM) complexes at the origin
of replication, which plays a vital role in both the initiation and elongation process of DNA
replication. The aim of this study is to establish M2-resistant cells over a period of time and
analyse different traits found in them, with hopes to further our knowledge on M2. Our data show
a correlation between chromatin-bound MCM level and S-phase progression speed, which was
consistently observed among the resistant population ‘R8’ and different clones. The results from
our study are in line with the known working mechanism of M2, and also hint at the importance
of the established resistant cells for future studies such as in investigating methods to overcome
resistance to M2.
Post a Comment