Finding a mating partner in a dioecious species is an obligatory process for species survival. We have used C. remanei and C. elegans as models to show that only females of the dioecious species can produce a volatile and stable sex pheromone to effectively attract males. Since males from both dioecious and androdioecious species respond to this same pheromone, I utilize these species to focus my investigation on three specific issues: To study sex pheromone production in C. remanei, to characterize C. remanei female sex pheromone, and to identify the cellular and the molecular components for sex pheromone perception....[ Read more ]

Finding a mating partner in a dioecious species is an obligatory process for species survival. We have used C. remanei and C. elegans as models to show that only females of the dioecious species can produce a volatile and stable sex pheromone to effectively attract males. Since males from both dioecious and androdioecious species respond to this same pheromone, I utilize these species to focus my investigation on three specific issues: To study sex pheromone production in C. remanei, to characterize C. remanei female sex pheromone, and to identify the cellular and the molecular components for sex pheromone perception.

I show by different combination of ablation on the gonadal precursor cells that the pheromone is produced by the somatic gonad. The production peaks at the young adult stage, and mating with males would eliminate the attractiveness of a female. Mating itself and the presence of sperms in the female body would not eliminate the pheromone attractiveness. The mechanism of mating on the attenuation of attractiveness will be discussed. While the perception of the pheromone can be delineated molecularly and cellularly using androdioecious C. elegans males, two different sensory neurons and an interneuron are shown to be required for this perception. A signaling pathway dependant of G-protein coupled kinase activity and G-protein subunit are crucial for this chemosensory response. The process may act through the gating of channels in specific neurons. The evaluation of the function of these molecules in specific neurons will be presented.