Microfluidic technology has been experiencing fast developing decades. It usually aims to offer better solutions for various areas, including chemical synthesis, analysis and biomedical science. Among all microfluidics materials, Poly(dimethylsiloxane) (PDMS) greatly simplifies microchips fabrication method and has been developed rapidly in this field. However, applications of PDMS chips are severely limited by a few drawbacks that are inherent to this material. Considering of Teflon's properties, such as gas permeable and bio-friendly, we applied thermobonding process to fabricating a device for different biological applications.

In this study, we have mainly focused on fabrication of multilayer Teflon chip and its biological applications. In the first part, we fabricated mult...[ Read more ]

Microfluidic technology has been experiencing fast developing decades. It usually aims to offer better solutions for various areas, including chemical synthesis, analysis and biomedical science. Among all microfluidics materials, Poly(dimethylsiloxane) (PDMS) greatly simplifies microchips fabrication method and has been developed rapidly in this field. However, applications of PDMS chips are severely limited by a few drawbacks that are inherent to this material. Considering of Teflon's properties, such as gas permeable and bio-friendly, we applied thermobonding process to fabricating a device for different biological applications.

In this study, we have mainly focused on fabrication of multilayer Teflon chip and its biological applications. In the first part, we fabricated multilayer Teflon chips and ensure stable samples infusion to simulate cell culture conditions. We further detected the performance of Teflon chips by testing the free radical-induced cytotoxicity and antioxidant-mediated viability. Then we designed a four-layer chip to explore the combination effect of various estrogens and estrogen receptor antagonists on MVLN cells. These inventions and new designs could help expand the potential applications of microfluidic devices.