THESIS
2015
iv leaves, v-x, 56 pages : illustrations (some color) ; 30 cm
Abstract
In yeast, Vps74p mediates the Golgi membrane retention of glycosyltransferases by
incorporating these enzymes into coat protein complex I (COPI) coated vesicles. VPS74 is not
an essential gene indicating that there must be a parallel pathway of VPS74 in the COPI
mediated traffic from Golgi to ER or within Golgi apparatus. Dr. TU in our lab found a vps74
temperature sensitive mutant. In order to find which genes may function in the parallel pathway
of Vps74p, I did a dosage suppressor screen of this vps74 temperature sensitive mutant and
found many genes could suppress the temperature sensitive growth phenotype of this vps74
strain. These dosage suppressors didn’t affect the retrograde transport of Kre2p and Dcr2p from
Golgi to ER. Only MAD1 among these suppressors could suppress...[
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In yeast, Vps74p mediates the Golgi membrane retention of glycosyltransferases by
incorporating these enzymes into coat protein complex I (COPI) coated vesicles. VPS74 is not
an essential gene indicating that there must be a parallel pathway of VPS74 in the COPI
mediated traffic from Golgi to ER or within Golgi apparatus. Dr. TU in our lab found a vps74
temperature sensitive mutant. In order to find which genes may function in the parallel pathway
of Vps74p, I did a dosage suppressor screen of this vps74 temperature sensitive mutant and
found many genes could suppress the temperature sensitive growth phenotype of this vps74
strain. These dosage suppressors didn’t affect the retrograde transport of Kre2p and Dcr2p from
Golgi to ER. Only MAD1 among these suppressors could suppress the calcofluor white
sensitivity of vps74 temperature sensitive mutant. In cells, there are two pools of Mad1p, one
is associated with Mad2p, and another is free. From our experiments, free Mad1p may have a
new role in protein traffic except for its function in spindle assembly checkpoint and may
localize to Golgi through interaction with Coy1p. In the future, I will keep on investigating the
reasons for the suppression of these dosage suppressors, and for Mad1p, I will make sure Mad1p
really localize in Golgi and find its cargoes if function in protein traffic.
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