Cell poration and intracellular delivery are significant works in the therapeutic and research fields. A lot of methods have been presented for cell poration. The cell penetrating peptides, transduction with viral vectors or membrane pore-forming agents have been used for the delivery of materials. But the specific demands in different targets and cargos make the preparation steps exclusive. Vector free methods such as microinjection, electroporation, and laser induce microbubble poration have got lots of progress in the delivery generalization. Without the limit of the reagents, the cell poration are controlled by the pipette penetrating, electric field and laser power. On the other hands, the harsh methods impair the cell's viability, which influence the overall efficiency.

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Cell poration and intracellular delivery are significant works in the therapeutic and research fields. A lot of methods have been presented for cell poration. The cell penetrating peptides, transduction with viral vectors or membrane pore-forming agents have been used for the delivery of materials. But the specific demands in different targets and cargos make the preparation steps exclusive. Vector free methods such as microinjection, electroporation, and laser induce microbubble poration have got lots of progress in the delivery generalization. Without the limit of the reagents, the cell poration are controlled by the pipette penetrating, electric field and laser power. On the other hands, the harsh methods impair the cell's viability, which influence the overall efficiency.

Here a microfluidic device for mechanoporation and cytosolic delivery is presented. The device contains micro- point constrictions featuring sharp edge formed in the silicon isotropic etching. Cell suspensions driven by the regulator controlled pressure are injected to the device. It is supposed that the cells passing by the constriction will experience high stress and strain, which leads to deformation and poration of the cells. The materials need to be delivered are contained in the cell suspension. Once the membrane pores generated, the materials diffuse into the cells' cytosol. We used the numerical modeling to study the flow and the cell in the microchannel and the constrictions. The stress and strain rate of the cells in the constriction suggest high possibility of cell poration. Experiments about delivery of dextran molecules, antibodies and RNA molecules have been done. And it is validated that the delivery method are applicable to different types of cells. The delivery performance are evaluated by microscopic and flow cytometer analyses. The satisfying efficiencies of delivery and transfection of wide range of materials to different types of cells prove the method' flexibility and generalization.