THESIS
2019
ix, 52 pages : color illustrations ; 30 cm
Abstract
Our lab has identified a novel group of ectoderm derived myeloid-like cell type in zebrafish epidermis.
We named them as metaphocytes based on their function to sample antigen from external
environment and then transport the antigen to epidermis-resident macrophage Langerhans cells[1].
Expect for the antigen transportation function, we are eager to explore more function of metaphocytes.
From our RNA-sequencing data and further characterization, we identified a metaphocytes
specific marker. I therefore generated two transgenic lines, in which the promoter of the metaphocyte
marker gene drives the expression of diphtheria toxin A (DTA) and nitroreductase (NTR) respectively.
The expression of DTA allows us to permanently deplete metaphocytes, whereas NTR
enables us to deplete metap...[
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Our lab has identified a novel group of ectoderm derived myeloid-like cell type in zebrafish epidermis.
We named them as metaphocytes based on their function to sample antigen from external
environment and then transport the antigen to epidermis-resident macrophage Langerhans cells[1].
Expect for the antigen transportation function, we are eager to explore more function of metaphocytes.
From our RNA-sequencing data and further characterization, we identified a metaphocytes
specific marker. I therefore generated two transgenic lines, in which the promoter of the metaphocyte
marker gene drives the expression of diphtheria toxin A (DTA) and nitroreductase (NTR) respectively.
The expression of DTA allows us to permanently deplete metaphocytes, whereas NTR
enables us to deplete metaphocytes upon MTZ administration. With the help of these two depletion
lines, we can characterize the functional diversities of metaphocytes.
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