The identification of pathogenic organism is essential to the prevention and distinguish the problems connected with health and safety. The growing public concern over the spread of the disease and the tough legislation in food industry made the failure to detect an infection might cause dreadful consequences. People are looking forward to obtaining the analytical results as soon as possible, the traditional pathogen detection protocol spend up to 6 to 10 days to yield an answer. Such long period is dissatisfactory, and many experts have geared their efforts towards the rapid, sensitivity methods. The availability of modern detection platforms plays a key role in the speed and accuracy of monitoring, surveillance, and quantitative infectious biological agent, and has a major influence o...[ Read more ]

The identification of pathogenic organism is essential to the prevention and distinguish the problems connected with health and safety. The growing public concern over the spread of the disease and the tough legislation in food industry made the failure to detect an infection might cause dreadful consequences. People are looking forward to obtaining the analytical results as soon as possible, the traditional pathogen detection protocol spend up to 6 to 10 days to yield an answer. Such long period is dissatisfactory, and many experts have geared their efforts towards the rapid, sensitivity methods. The availability of modern detection platforms plays a key role in the speed and accuracy of monitoring, surveillance, and quantitative infectious biological agent, and has a major influence on enacts regulations to promote the best practices to prevent pathophoresis.

In the past four years, my studies were basically focused on developing convenient and effective isothermal amplification based methods for rapid pathogen detection and combining different techniques for nucleic acid quantitative analysis. On the basis of developed isothermal amplification detection technique, I explored its applications in POCT device, which exhibited great potentials in the electricity-free and equipment–free pathogen detection in field. Also, microfluidic technology minimized the POCT device with the help of novel valve design.

In the first chapter, an overview of POCT device for nucleic acid analysis devices including their significance, current advances and challenges are present. In the rest chapters, I present the research projects completed during my PhD study, including development of real-time recombinase polymerase amplification (RPA) assay for rapid detection of Herpes simplex virus-1 (HSV-1) in human tears, POCT in the field: a hand-powered and sample-in-answer-out device (HASDE) for nucleic acid detection without the requirement of electrical supply and extra equipment and a new paradigm for valve design in microfluidic chip for the facile isothermal sample-in-answer-out nucleic acid identification.