THESIS
1996
xiv, 68 leaves : ill., photos. (some col.) ; 30 cm
Abstract
Mechanical trauma and cerebral ischemia are two major types of injury in the central nervous system. After sujected to these injuries, brain would develop immune response. It is known that the proinflammatory cytokines - interleukin 1 (IL- lα), tumour necrosis factor a (TNFα), interleukin 6 (IL-6) and interferon γ (IFNγ) are responsible for triggering immune response. Due to the presence of the blood brain barrier, resident brain cells were thought to be the early sources of these cytokines. Therefore, the expression of these cytokines in astrocyte, one of the major cell types in brain, was studied under either mechanical or ischemic injury. Because of the complexity of the brain, two in vitro injury models were created using confluent primary cultures of astrocyte. One was the scratch...[
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Mechanical trauma and cerebral ischemia are two major types of injury in the central nervous system. After sujected to these injuries, brain would develop immune response. It is known that the proinflammatory cytokines - interleukin 1 (IL- lα), tumour necrosis factor a (TNFα), interleukin 6 (IL-6) and interferon γ (IFNγ) are responsible for triggering immune response. Due to the presence of the blood brain barrier, resident brain cells were thought to be the early sources of these cytokines. Therefore, the expression of these cytokines in astrocyte, one of the major cell types in brain, was studied under either mechanical or ischemic injury. Because of the complexity of the brain, two in vitro injury models were created using confluent primary cultures of astrocyte. One was the scratch wound model and the other was the anaerobic chamber induced ischemic model. In the scratch wound model, cells along the scratch showed positive staining to IL- lα TNF[alpha][alpha] and IL-6 antibodies. No cells could be stained by IFNγ antibody. By using double immunostaining, astrocyte was shown to express IL-lα and IL-6. As the cytokines are secretory proteins, the changes in the levels of these cytokines in the culture media were also measured. The levels of these cytokines increased with time after scratch. In the ischemic model, the gene expressions of IL-lα, TNF[alpha][alpha] and IL-6 were enhanced. Also, all of the proinflammatory cytokines increased in the media of the cultured astrocyte under ischemic injury. This study provided information on the expressions of the proinflammatory cytokines after mechanical injury and ischemic injury. This information would help to understand the development of brain immune response after injury.
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